Several Founding Fathers discussed inalienable or unalienable rights (these words were used interchangeably) in their writings. See here for more on this issue.
Many of the 56 Declaration signers encountered significant hardships including death, losing loved ones, and the destruction of their property. Read more about these difficulties and sacrifices here.
A few Declaration of Independence signers were active in the ministry at that time. Read more about this here.
Some Founding Fathers did own slaves but many strongly and actively opposed slavery. Read more about this topic here.
More than 250 individuals qualify as Founding Fathers, and the majority of them were Christians. See more information on this topic here.